Ontology highlight
ABSTRACT:
SUBMITTER: Jacques-Silva MC
PROVIDER: S-EPMC2851966 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Jacques-Silva M Caroline MC Correa-Medina Mayrin M Cabrera Over O Rodriguez-Diaz Rayner R Makeeva Natalia N Fachado Alberto A Diez Juan J Berman Dora M DM Kenyon Norma S NS Ricordi Camillo C Pileggi Antonello A Molano R Damaris RD Berggren Per-Olof PO Caicedo Alejandro A
Proceedings of the National Academy of Sciences of the United States of America 20100322 14
Extracellular ATP has been proposed as a paracrine signal in rodent islets, but it is unclear what role ATP plays in human islets. We now show the presence of an ATP signaling pathway that enhances the human beta cell's sensitivity and responsiveness to glucose fluctuations. By using in situ hybridization, RT-PCR, immunohistochemistry, and Western blotting as well as recordings of cytoplasmic-free Ca(2+) concentration, [Ca(2+)](i), and hormone release in vitro, we show that human beta cells expr ...[more]