Auxin polar transport is essential for the development of zygote and embryo in Nicotiana tabacum L. and correlated with ABP1 and PM H+-ATPase activities.

ABSTRACT: Auxin is an important plant growth regulator, and plays a key role in apical-basal axis formation and embryo differentiation, but the mechanism remains unclear. The level of indole-3-acetic acid (IAA) during zygote and embryo development of Nicotiana tabacum L. is investigated here using the techniques of GC-SIM-MS analysis, immunolocalization, and the GUS activity assay of DR5::GUS transgenic plants. The distribution of ABP1 and PM H(+)-ATPase was also detected by immunolocalization, and this is the first time that integral information has been obtained about their distribution in the zygote and in embryo development. The results showed an increase in IAA content in ovules and the polar distribution of IAA, ABP1, and PM H(+)-ATPase in the zygote and embryo, specifically in the top and bas

SUBMITTER: Chen D 

PROVIDER: S-EPMC2852673 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature