A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI.

ABSTRACT: Insight into protein structure and function is best obtained through a synthesis of experimental, structural and bioinformatic data. Here, we outline a framework that we call MUSE (mutual information, unigenic evolution and structure-guided elucidation), which facilitated the identification of previously unknown residues that are relevant for function of the GIY-YIG homing endonuclease I-BmoI. Our approach synthesizes three types of data: mutual information analyses that identify co-evolving residues within the GIY-YIG catalytic domain; a unigenic evolution strategy that identifies hyper- and hypo-mutable residues of I-BmoI; and interpretation of the unigenic and co-evolution data using a homology model. In particular, we identify novel positions within the GIY-YIG domain as functionally i

SUBMITTER: Kleinstiver BP 

PROVIDER: S-EPMC2853131 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature