Oncosome formation in prostate cancer: association with a region of frequent chromosomal deletion in metastatic disease.

ABSTRACT: Oncosomes have recently been described as membrane-derived microvesicles secreted by cancer cells, which transfer oncogenic signals and protein complexes across cell boundaries. Here, we show the rapid formation and secretion of oncosomes from DU145 and LNCaP human prostate cancer cells. Oncosome formation was stimulated by epidermal growth factor receptor activation and also by overexpression of membrane-targeted Akt1. Microvesicles shed from prostate cancer cells contained numerous signal transduction proteins and were capable of activating rapid phospho-tyrosine and Akt pathway signaling, and stimulating proliferation and migration, in recipient tumor cells. They also induced a stromal reaction in recipient normal cells. Knockdown of the actin nucleating protein Diaphanous Related Formi

SUBMITTER: Di Vizio D 

PROVIDER: S-EPMC2853876 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature