MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation.

ABSTRACT: MicroRNAs (miRNAs) are non-protein coding molecules with important regulatory functions; many have tissue-specific expression patterns. Their very small size in principle makes them less prone to degradation processes, unlike messenger RNAs (mRNAs), which were previously proposed as molecular tools for forensic body fluid identification. To identify suitable miRNA markers for forensic body fluid identification, we first screened total RNA samples derived from saliva, semen, vaginal secretion, and venous and menstrual blood for the expression of 718 human miRNAs using a microarray platform. All body fluids could be easily distinguished from each other on the basis of complete array-based miRNA expression profiles. Results from quantitative reverse transcription PCR (RT-PCR; TaqMan) assays f

SUBMITTER: Zubakov D 

PROVIDER: S-EPMC2855015 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature