Analysis of actin FLAP dynamics in the leading lamella.

ABSTRACT:

Background

The transport of labeled G-actin from the mid-lamella region to the leading edge in a highly motile malignant rat fibroblast line has been studied using fluorescence localization after photobleaching or FLAP, and the transit times recorded in these experiments were so fast that simple diffusion was deemed an insufficient explanation (see Zicha et al., Science, v. 300, pp. 142-145 [1]).

Methodology/principal findings

We re-examine the Zicha FLAP experiments using a two-phase reactive interpenetrating flow formalism to model the cytoplasm and the transport dynamics of bleached and unbleached actin. By allowing an improved treatment of effects related to the retrograde flow of the cytoskeleton and of the geometry and finite thickness of the lamella, this new analysis

SUBMITTER: Kuznetsov IR 

PROVIDER: S-EPMC2855347 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature