Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium.

ABSTRACT:

Purpose

Hydration and ionic composition of the subretinal space (SRS) is modulated by the retinal pigment epithelium (RPE). In particular calcium concentration (Ca(2+)) in the SRS varies with light exposure, and although this change is regulated by RPE transport activity, the specific transport proteins involved have yet to be defined. Two members of the transient receptor potential vanilloid family, TRPV5 and TRPV6, are calcium selective ion channels and are known to be expressed in calcium-transporting epithelial tissues. The present work characterizes of TRPV5 and TRPV6 in RPE.

Methods

Reverse transcriptase PCR was used to examine the presence of TRPV5 and TRPV6 mRNA in cultured human RPE. Protein expression was assessed by western blotting using TRPV5- and TRPV6-specific

SUBMITTER: Kennedy BG 

PROVIDER: S-EPMC2855730 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature