Comparative analysis of conditional reporter alleles in the developing embryo and embryonic nervous system.

ABSTRACT: A long-standing problem in development is understanding how progenitor cells transiently expressing genes contribute to complex anatomical and functional structures. In the developing nervous system an additional level of complexity arises when considering how cells of distinct lineages relate to newly established neural circuits. To address these problems, we used both cumulative marking with Cre/loxP and Genetic Inducible Fate Mapping (GIFM), which permanently and heritably marks small populations of progenitors and their descendants with fine temporal control using CreER/loxP. A key component used in both approaches is a conditional phenotyping allele that has the potential to be expressed in all cell types, but is quiescent because of a loxP flanked Stop sequence, which precedes a repo

SUBMITTER: Ellisor D 

PROVIDER: S-EPMC2855890 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature