PCR-based method for targeting 16S-23S rRNA intergenic spacer regions among Vibrio species.

ABSTRACT:

Background

The genus Vibrio is a diverse group of Gram-negative bacteria comprised of 74 species. Furthermore, the genus has and is expected to continue expanding with the addition of several new species annually. Consequently, it is of paramount importance to have a method which is able to reliably and efficiently differentiate the numerous Vibrio species.

Results

In this study, a novel and rapid polymerase chain reaction (PCR)-based intergenic spacer (IGS)-typing system for vibrios was developed that is based on the well-known IGS regions located between the 16S and 23S rRNA genes on the bacterial chromosome. The system was optimized to resolve heteroduplex formation as well as to take advantage of capillary gel electrophoresis technology such that reproducible analyses co

SUBMITTER: Hoffmann M 

PROVIDER: S-EPMC2856557 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature