A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts.

ABSTRACT:

Background

Current diagnostics for allergies, such as skin prick and radioallergosorbent tests, do not allow for inexpensive, high-throughput screening of patients. Additionally, extracts used in these methods are made from washed pollen that lacks pollen surface materials that may contain allergens.

Methodology/principal findings

We sought to develop a high-throughput assay to rapidly measure allergen-specific IgE in sera and to explore the relative allergenicity of different pollen fractions (i.e. surface, cytoplasmic, commercial extracts). To do this, we generated a protein microarray containing surface, cytoplasmic, and commercial extracts from 22 pollen species, commercial extracts from nine non-pollen allergens, and five recombinant allergenic proteins. Pollen surface

SUBMITTER: Vigh-Conrad KA 

PROVIDER: S-EPMC2856625 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature