Salivary proteomic and genomic biomarkers for primary Sjogren's syndrome.

ABSTRACT:

Objective

To identify a panel of protein and messenger RNA (mRNA) biomarkers in human whole saliva (WS) that may be used in the detection of primary Sjögren's syndrome (SS).

Methods

Mass spectrometry and expression microarray profiling were used to identify candidate protein and mRNA biomarkers of primary SS in WS samples. Validation of the discovered mRNA and protein biomarkers was also demonstrated using real-time quantitative polymerase chain reaction and immunoblotting techniques.

Results

Sixteen WS proteins were found to be down-regulated and 25 WS proteins were found to be up-regulated in primary SS patients compared with matched healthy control subjects. These proteins reflected the damage of glandular cells and inflammation of the oral cavity system in patient

SUBMITTER: Hu S 

PROVIDER: S-EPMC2856841 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature