Dynamic regulation of CD45 lateral mobility by the spectrin-ankyrin cytoskeleton of T cells.

ABSTRACT: The leukocyte common antigen, CD45, is a critical immune regulator whose activity is modulated by cytoskeletal interactions. Components of the spectrin-ankyrin cytoskeleton have been implicated in the trafficking and signaling of CD45. We have examined the lateral mobility of CD45 in resting and activated T lymphocytes using single-particle tracking and found that the receptor has decreased mobility caused by increased cytoskeletal contacts in activated cells. Experiments with cells that have disrupted betaI spectrin interactions show decreased cytoskeletal contacts in resting cells and attenuation of receptor immobilization in activated cells. Applying two types of population analyses to single-particle tracking trajectories, we find good agreement between the diffusion coefficients obtai

SUBMITTER: Cairo CW 

PROVIDER: S-EPMC2857017 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature