Determinants for stop-transfer and post-import pathways for protein targeting to the chloroplast inner envelope membrane.

ABSTRACT: The inner envelope membrane (IEM) of the chloroplast plays key roles in controlling metabolite transport between the organelle and cytoplasm and is a major site of lipid and membrane synthesis within the organelle. IEM biogenesis requires the import and integration of nucleus-encoded membrane proteins. Previous reports have led to the conclusion that membrane proteins are inserted into the IEM during protein import from the cytoplasm via a stop-transfer mechanism or are completely imported into the stroma and then inserted into the IEM in a post-import mechanism. In this study, we examined the determinants for each pathway by comparing the targeting of APG1 (albino or pale green mutant 1), an example of a stop-transfer substrate, and atTic40, an example of a post-import substrate. We show

SUBMITTER: Viana AA 

PROVIDER: S-EPMC2857071 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature