Transforming growth factor beta3 regulates the versican variants in the extracellular matrix-rich uterine leiomyomas.

ABSTRACT: Uterine leiomyoma are common, benign tumors that are enriched in extracellular matrix. The tumors are characterized by a disoriented and loosely packed collagen fibril structure similar to other diseases with disrupted Transforming growth factor beta (TGF-beta) signaling. Here we characterized TGF-beta3 signaling and the expression patterns of the critical extracellular matrix component versican in leiomyoma and myometrial tissue and cell culture. We also demonstrate the regulation of the versican variants by TGF-beta3. Using leiomyoma and matched myometrium from 15 patients, messenger RNA (mRNA) from leiomyoma and myometrium was analyzed by semiquantitative real time reverse transcription-polymerase chain reaction (RT-PCR), while protein analysis was done by western blot. Transforming gro

SUBMITTER: Norian JM 

PROVIDER: S-EPMC2857408 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature