A novel mitochondrial K(ATP) channel assay.

ABSTRACT:

Rationale

The mitochondrial ATP sensitive potassium channel (mK(ATP)) is implicated in cardioprotection by ischemic preconditioning (IPC), but the molecular identity of the channel remains controversial. The validity of current methods to assay mK(ATP) activity is disputed.

Objective

We sought to develop novel methods to assay mK(ATP) activity and its regulation.

Methods and results

Using a thallium (Tl(+))-sensitive fluorophore, we developed a novel Tl(+) flux based assay for mK(ATP) activity, and used this assay probe several aspects of mK(ATP) function. The following key observations were made. (1) Time-dependent run down of mK(ATP) activity was reversed by phosphatidylinositol-4,5-bisphosphate (PIP(2)). (2) Dose responses of mK(ATP) to nucleotides revealed a UDP E

SUBMITTER: Wojtovich AP 

PROVIDER: S-EPMC2857559 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature