Novel snail1 target proteins in human colon cancer identified by proteomic analysis.

ABSTRACT:

Background

The transcription factor Snail1 induces epithelial-to-mesenchymal transition (EMT), a process responsible for the acquisition of invasiveness during tumorigenesis. Several transcriptomic studies have reported Snail1-regulated genes in different cell types, many of them involved in cell adhesion. However, only a few studies have used proteomics as a tool for the characterization of proteins mediating EMT.

Methodology/principal findings

We identified by proteomic analysis using 2D-DIGE electrophoresis combined with MALDI-TOF-TOF and ESI-linear ion trap mass spectrometry a number of proteins with variable functions whose expression is modulated by Snail1 in SW480-ADH human colon cancer cells. Validation was performed by Western blot and immunofluorescence analyses. S

SUBMITTER: Larriba MJ 

PROVIDER: S-EPMC2857666 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature