Dendritic cell podosomes are protrusive and invade the extracellular matrix using metalloproteinase MMP-14.

ABSTRACT: Podosomes are spot-like actin-rich structures formed at the ventral surface of monocytic and haematopoietic cells. Podosomes degrade extracellular matrix and are proposed to be involved in cell migration. A key question is whether podosomes form protrusions similar to the invadopodia of cancer cells. We characterised podosomes of immature dendritic cells using electron microscopy combined with both conventional and novel high-resolution structured illumination light microscopy. Dendritic cell podosomes are composed of actin foci surrounded by a specialised ring region that is rich in material containing paxillin. We found that podosomes were preferential sites for protrusion into polycarbonate filters impregnated with crosslinked gelatin, degrading up to 2 micrometers of matrix in 24 hours

SUBMITTER: Gawden-Bone C 

PROVIDER: S-EPMC2858019 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature