Transcriptome analysis of the Brucella abortus BvrR/BvrS two-component regulatory system.

ABSTRACT:

Background

The two-component BvrR/BvrS system is essential for Brucella abortus virulence. It was shown previously that its dysfunction alters the expression of some major outer membrane proteins and the pattern of lipid A acylation. To determine the genes regulated by BvrR/BvrS, we performed a whole-genome microarray analysis using B. abortus RNA obtained from wild type and bvrR mutant cells grown in the same conditions.

Methodology/principal findings

A total of 127 differentially expressed genes were found: 83 were over expressed and 44 were less expressed in the bvrR mutant. Two operons, the phosphotransferase system and the maltose transport system, were down-regulated. Several genes involved in cell envelope or outer membrane biogenesis were differentially expressed: ge

SUBMITTER: Viadas C 

PROVIDER: S-EPMC2858072 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature