GINS motion reveals replication fork progression is remarkably uniform throughout the yeast genome.

ABSTRACT: Previous studies have led to a picture wherein the replication of DNA progresses at variable rates over different parts of the budding yeast genome. These prior experiments, focused on production of nascent DNA, have been interpreted to imply that the dynamics of replication fork progression are strongly affected by local chromatin structure/architecture, and by interaction with machineries controlling transcription, repair and epigenetic maintenance. Here, we adopted a complementary approach for assaying replication dynamics using whole genome time-resolved chromatin immunoprecipitation combined with microarray analysis of the GINS complex, an integral member of the replication fork. Surprisingly, our data show that this complex progresses at highly uniform rates regardless of genomic loc

SUBMITTER: Sekedat MD 

PROVIDER: S-EPMC2858444 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature