Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse.

ABSTRACT:

Background

IZUMO1 is the only sperm protein which is proven to be essential for sperm-egg fusion. However, the IZUMO1 is a structurally simple protein with single Ig domain and seems not to include either a "fusogenic peptide" or a fusion machinery domain. This led us to assume the existence of an IZUMO1-interacting protein(s) which makes a functional fusion machine interacting with IZUMO1.

Methodology/principal findings

We produced a transgenic mouse line which expresses His-tagged IZUMO1 in the Izumo1(-/-) genetic background. After solubilization of sperm membranes, we purified His-tagged IZUMO1 using anti-His affinity chromatography and found a protein that interacts with IZUMO1. After being separated on SDS-PAGE gel, the IZUMO1-interacting protein was subjected to LC-MS/

SUBMITTER: Inoue N 

PROVIDER: S-EPMC2858660 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature