Oligonucleotide microarray for the identification of potential mycotoxigenic fungi.

ABSTRACT:

Background

Mycotoxins are secondary metabolites which are produced by numerous fungi and pose a continuous challenge to the safety and quality of food commodities in South Africa. These toxins have toxicologically relevant effects on humans and animals that eat contaminated foods. In this study, a diagnostic DNA microarray was developed for the identification of the most common food-borne fungi, as well as the genes leading to toxin production.

Results

A total of 40 potentially mycotoxigenic fungi isolated from different food commodities, as well as the genes that are involved in the mycotoxin synthetic pathways, were analyzed. For fungal identification, oligonucleotide probes were designed by exploiting the sequence variations of the elongation factor 1-alpha (EF-1 alpha) c

SUBMITTER: Lezar S 

PROVIDER: S-EPMC2858739 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature