Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device.

ABSTRACT: We demonstrate an optical Fourier processing method to quantify object texture arising from subcellular feature orientation within unstained living cells. Using a digital micromirror device as a Fourier spatial filter, we measured cellular responses to two-dimensional optical Gabor-like filters optimized to sense orientation of nonspherical particles, such as mitochondria, with a width around 0.45 microm. Our method showed significantly rounder structures within apoptosis-defective cells lacking the proapoptotic mitochondrial effectors Bax and Bak, when compared with Bax/Bak expressing cells functional for apoptosis, consistent with reported differences in mitochondrial shape in these cells. By decoupling spatial frequency resolution from image resolution, this method enables rapid analysi

SUBMITTER: Pasternack RM 

PROVIDER: S-EPMC2859170 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature