USE OF MULTIPLE SINGULAR VALUE DECOMPOSITIONS TO ANALYZE COMPLEX INTRACELLULAR CALCIUM ION SIGNALS.

ABSTRACT: We compare calcium ion signaling (Ca(2+)) between two exposures; the data are present as movies, or, more prosaically, time series of images. This paper describes novel uses of singular value decompositions (SVD) and weighted versions of them (WSVD) to extract the signals from such movies, in a way that is semi-automatic and tuned closely to the actual data and their many complexities. These complexities include the following. First, the images themselves are of no interest: all interest focuses on the behavior of individual cells across time, and thus, the cells need to be segmented in an automated manner. Second, the cells themselves have 100+ pixels, so that they form 100+ curves measured over time, so that data compression is required to extract the features of these curves. Third, som

SUBMITTER: Martinez JG 

PROVIDER: S-EPMC2859732 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature