Gene expression profiling following short-term and long-term morphine exposure in mice uncovers genes involved in food intake.

ABSTRACT: Addictive drugs including opioids activate signal transduction pathways that regulate gene expression in the brain. However, changes in CNS gene expression following morphine exposure are poorly understood. We determined changes in gene expression following short- and long-term morphine treatment in the hypothalamus and pituitary using genome-wide DNA microarray analysis and confirmed those alterations in gene expression by real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis. In the hypothalamus, short-term morphine administration up-regulated (at least twofold) 39 genes and down-regulated six genes. Long-term morphine treatment up-regulated 35 genes and down-regulated 51 genes. In the pituitary, short-term morphine administration up-regulated 110 genes and down-reg

SUBMITTER: Anghel A 

PROVIDER: S-EPMC2859888 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature