Analysis of Arabidopsis thaliana root growth kinetics with high temporal and spatial resolution.

ABSTRACT:

Background

Methods exist to quantify the distribution of growth rate over the root axis. However, non-destructive, high-throughput evaluations of total root elongation in controlled environments and the field are lacking in growth studies. A new imaging approach to analyse total root elongation is described.

Scope

High pixel resolution of the images enables the study of growth in short time intervals and provides high temporal resolution. Using the method described, total root elongation rates are calculated from the displacement of the root tip. Although the absolute root elongation rate changes in response to growth conditions, this set-up enables root growth of Arabidopsis wild-type seedlings to be followed for more than 1 month after germination. The method provides an e

SUBMITTER: Yazdanbakhsh N 

PROVIDER: S-EPMC2859919 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature