Protein evolution via amino acid and codon elimination.

ABSTRACT:

Background

Global residue-specific amino acid mutagenesis can provide important biological insight and generate proteins with altered properties, but at the risk of protein misfolding. Further, targeted libraries are usually restricted to a handful of amino acids because there is an exponential correlation between the number of residues randomized and the size of the resulting ensemble. Using GFP as the model protein, we present a strategy, termed protein evolution via amino acid and codon elimination, through which simplified, native-like polypeptides encoded by a reduced genetic code were obtained via screening of reduced-size ensembles.

Methodology/principal findings

The strategy involves combining a sequential mutagenesis scheme to reduce library size with structurally s

SUBMITTER: Goltermann L 

PROVIDER: S-EPMC2859931 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature