Effect of oxymatrine on the replication cycle of hepatitis B virus in vitro.

ABSTRACT:

Aim

To determine the antiviral mechanism or target of oxymatrine against hepatitis B virus (HBV).

Methods

HepG2.2.15 cells were incubated with culture medium containing 500 microg/mL of oxymatrine for 2 and 5 d. The surface antigen of HBV (HBsAg) and e antigen of HBV (HBeAg) in supernatant were determined by ELISA. HBV DNA in supernatant, and intracellular covalently closed circular DNA (cccDNA), relaxed circular DNA (rcDNA) and pregenomic RNA (pgRNA) were quantified by specific real-time polymerase chain reaction (PCR) or reverse transcription (RT)-PCR.

Results

Treatment with oxymatrine for 2 d and 5 d reduced the production of HBV by the cell line, as indicated by the decline of HBsAg (22.67%, t = 5.439, P = 0.0322 and 22.39%, t = 5.376, P = 0.0329, respectively), H

SUBMITTER: Xu WS 

PROVIDER: S-EPMC2860082 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature