Histone H1 variant-specific lysine methylation by G9a/KMT1C and Glp1/KMT1D.

ABSTRACT:

Background

The linker histone H1 has a key role in establishing and maintaining higher order chromatin structure and in regulating gene expression. Mammals express up to 11 different H1 variants, with H1.2 and H1.4 being the predominant ones in most somatic cells. Like core histones, H1 has high levels of covalent modifications; however, the full set of modifications and their biological role are largely unknown.

Results

In this study, we used a candidate screen to identify enzymes that methylate H1 and to map their corresponding methylation sites. We found that the histone lysine methyltransferases G9a/KMT1C and Glp1/KMT1D methylate H1.2 in vitro and in vivo, and we mapped this novel site to lysine 187 (H1.2K187) in the C-terminus of H1. This H1.2K187 methylation is variant

SUBMITTER: Weiss T 

PROVIDER: S-EPMC2860349 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature