Intratumor heterogeneity and precision of microarray-based predictors of breast cancer biology and clinical outcome.

ABSTRACT:

Purpose

Identifying sources of variation in expression microarray data and the effect of variance in gene expression measurements on complex predictive and diagnostic models is essential when translating microarray-based experimental approaches into clinical assays. The technical reproducibility of microarray platforms is well established. Here, we investigate the additional impact of intratumor heterogeneity, a largely unstudied component of variance, on the performance of several microarray-based assays in breast cancer.

Patients and methods

Genome-wide expression profiling was performed on 50 core needle biopsies from 18 breast cancer patients using Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays. Global profiles of expression were characterized using unsupervised c

SUBMITTER: Barry WT 

PROVIDER: S-EPMC2860437 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature