Protein kinase C delta mediates arterial injury responses through regulation of vascular smooth muscle cell apoptosis.

ABSTRACT:

Aims

A balance between apoptosis and proliferation of vascular smooth muscle cells (VSMC) influences the development of intimal hyperplasia. We have previously demonstrated that protein kinase C delta (PKCdelta) regulates both apoptosis and proliferation of VSMC in vitro. Here we investigate the role of PKCdelta in intimal hyperplasia through gene deletion or overexpression in rodent models of arterial injury.

Methods and results

Arterial injury was induced in mice and rats by means of carotid ligation or balloon angioplasty, respectively. Overexpression of PKCdelta was achieved by adenovirus-mediated gene transfer immediately after balloon injury in rat carotid arteries. Levels of PKCdelta protein were profoundly increased in the carotid wall 3-7 days after balloon injury,

SUBMITTER: Yamanouchi D 

PROVIDER: S-EPMC2860709 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature