Identification of 4-deoxythreonic acid present in human urine using HPLC and NMR techniques.

ABSTRACT: The 1H NMR spectrum of urine exhibits a large number of detectable and quantifiable metabolites and hence urine metabolite profiling is potentially useful for the study of systems biology and the discovery of biomarkers for drug development or clinical applications. While a number of metabolites (50-100) are readily detectable in urine by NMR, a much larger number is potentially available if lower concentration species can be detected unambiguously. Lower concentration metabolites are thought to be more specific to certain disease states and thus it is important to detect these metabolites with certainty. We report the identification of 4-deoxythreonic acid, a relatively low concentration endogenous metabolite that has not been previously identified in the 1H NMR spectrum of human urine. T

SUBMITTER: Appiah-Amponsah E 

PROVIDER: S-EPMC2861046 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature