Spatial distribution of factor Xa, thrombin, and fibrin(ogen) on thrombi at venous shear.

ABSTRACT:

Background

The generation of thrombin is a critical process in the formation of venous thrombi. In isolated plasma under static conditions, phosphatidylserine (PS)-exposing platelets support coagulation factor activation and thrombin generation; however, their role in supporting coagulation factor binding under shear conditions remains unclear. We sought to determine where activated factor X (FXa), (pro)thrombin, and fibrin(ogen) are localized in thrombi formed under venous shear.

Methodology/principal findings

Fluorescence microscopy was used to study the accumulation of platelets, FXa, (pro)thrombin, and fibrin(ogen) in thrombi formed in vitro and in vivo. Co-perfusion of human blood with tissue factor resulted in formation of visible fibrin at low, but not at high shear r

SUBMITTER: Berny MA 

PROVIDER: S-EPMC2861630 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature