Decreased fibronectin production significantly contributes to dysregulated repair of asthmatic epithelium.

ABSTRACT:

Rationale

Damage to airway epithelium is followed by deposition of extracellular matrix (ECM) and migration of adjacent epithelial cells. We have shown that epithelial cells from children with asthma fail to heal a wound in vitro.

Objectives

To determine whether dysregulated ECM production by the epithelium plays a role in aberrant repair in asthma.

Methods

Airway epithelial cells (AEC) from children with asthma (n = 36), healthy atopic control subjects (n = 23), and healthy nonatopic control subjects (n = 53) were investigated by microarray, gene expression and silencing, transcript regulation analysis, and ability to close mechanical wounds.

Measurements and main results

Time to repair a mechanical wound in vitro by AEC from healthy and atopic children was no

SUBMITTER: Kicic A 

PROVIDER: S-EPMC2862303 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature