Pulsed direct current electric fields enhance osteogenesis in adipose-derived stromal cells.

ABSTRACT: Adipose-derived stromal cells (ASCs) constitute a promising source of cells for regenerative medicine applications. Previous studies of osteogenic potential in ASCs have focused on chemicals, growth factors, and mechanical stimuli. Citing the demonstrated role electric fields play in enhancing healing in bone fractures and defects, we investigated the ability of pulsed direct current electric fields to drive osteogenic differentiation in mouse ASCs. Employing 50 Hz direct current electric fields in concert with and without osteogenic factors, we demonstrated increased early osteoblast-specific markers. We were also able to establish that commonly reported artifacts of electric field stimulation are not the primary mediators of the observed effects. The electric fields caused marked changes

SUBMITTER: Hammerick KE 

PROVIDER: S-EPMC2862617 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature