Supporting cell division is not required for regeneration of auditory hair cells after ototoxic injury in vitro.

ABSTRACT: In chickens, nonsensory supporting cells divide and regenerate auditory hair cells after injury. Anatomical evidence suggests that supporting cells can also transdifferentiate into hair cells without dividing. In this study, we characterized an organ culture model to study auditory hair cell regeneration, and we used these cultures to test if direct transdifferentiation alone can lead to significant hair cell regeneration. Control cultures (organs from posthatch chickens maintained without streptomycin) showed complete hair cell loss in the proximal (high-frequency) region by 5 days. In contrast, a 2-day treatment with streptomycin induced loss of hair cells from all regions by 3 days. Hair cell regeneration proceeded in culture, with the time course of supporting cell division and hair ce

SUBMITTER: Shang J 

PROVIDER: S-EPMC2862922 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature