Analysis of Lactobacillus sakei mutants selected after adaptation to the gastrointestinal tracts of axenic mice.

ABSTRACT: We recently showed that Lactobacillus sakei, a natural meat-borne lactic acid bacterium, can colonize the gastrointestinal tracts (GIT) of axenic mice but that this colonization in the intestinal environment selects L. sakei mutants showing modified colony morphology (small and rough) and cell shape, most probably resulting from the accumulation of various mutations that confer a selective advantage for persistence in the GIT. In the present study, we analyzed such clones, issued from three different L. sakei strains, in order to determine which functions were modified in the mutants. In the elongated filamentous cells of the rough clones, transmission electron microscopy (TEM) analysis showed a septation defect and dotted and slanted black bands, suggesting the presence of a helical struc

SUBMITTER: Chiaramonte F 

PROVIDER: S-EPMC2863443 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature