Identification, characterization, and regulation of a novel antifungal chitosanase gene (cho) in Anabaena spp.

ABSTRACT: Two contrasting cyanobacterial species (Anabaena fertilissima and Anabaena sphaerica) were selected based on differences in antifungal behavior in order to study the mechanism for production of an antifungal enzyme and the genes responsible for this production. In A. fertilissima, chitosanase and antifungal activities were increased significantly under of growth-limiting conditions (8 of light and 16 h of darkness). The lack of such activities in A. sphaerica was associated with high levels of protein that accumulated during the stationary phase (at 28 days) under the same light conditions. The gene putatively responsible for chitosanase and antifungal activities was amplified using specific primers, and sequence analysis of the amplified products (1.086 and 1.101 kb in A. sphaerica and A.

SUBMITTER: Gupta V 

PROVIDER: S-EPMC2863463 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature