Transcriptome analysis of Pseudomonas syringae identifies new genes, noncoding RNAs, and antisense activity.

ABSTRACT: To fully understand how bacteria respond to their environment, it is essential to assess genome-wide transcriptional activity. New high-throughput sequencing technologies make it possible to query the transcriptome of an organism in an efficient unbiased manner. We applied a strand-specific method to sequence bacterial transcripts using Illumina's high-throughput sequencing technology. The resulting sequences were used to construct genome-wide transcriptional profiles. Novel bioinformatics analyses were developed and used in combination with proteomics data for the qualitative classification of transcriptional activity in defined regions. As expected, most transcriptional activity was consistent with predictions from the genome annotation. Importantly, we identified and confirmed transcrip

SUBMITTER: Filiatrault MJ 

PROVIDER: S-EPMC2863471 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature