Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas.

ABSTRACT: Retroviruses and retrovirus-derived vectors integrate nonrandomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. However, the genomic features that influence the transcriptional activities of integrated retroviruses or retroviral vectors are poorly understood. We report here the cloning and characterization of avian sarcoma virus integration sites from chicken tumors. Growing progressively, dependent on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Therefore, integration sites in our study distinguished genomic loci favorable for the expression of integrated retroviruses and gene transfer vec

SUBMITTER: Plachy J 

PROVIDER: S-EPMC2863777 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature