Binding site on the transferrin receptor for the parvovirus capsid and effects of altered affinity on cell uptake and infection.

ABSTRACT: Canine parvovirus (CPV) and its relative feline panleukopenia virus (FPV) bind the transferrin receptor type 1 (TfR) to infect their host cells but show differences in the interactions with the feline and canine TfRs that determine viral host range and tissue tropism. We changed apical and protease-like domain residues by introducing point mutations and adding or removing glycosylation signals, and we then examined the interactions of those mutant TfRs with the capsids. Most substitutions had little effect on virus binding and uptake. However, mutations of several sites in the apical domain of the receptor either prevented binding to the capsids or reduced the affinity of receptor binding to various degrees. Glycans within the virus binding face of the apical domain also controlled capsid

SUBMITTER: Goodman LB 

PROVIDER: S-EPMC2863798 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature