Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa.

ABSTRACT:

Background

The constant increase in development and spread of bacterial resistance to antibiotics poses a serious threat to human health. New sequencing technologies are now on the horizon that will yield massive increases in our capacity for DNA sequencing and will revolutionize the drug discovery process. Since essential genes are promising novel antibiotic targets, the prediction of gene essentiality based on genomic information has become a major focus.

Results

In this study we demonstrate that pooled sequencing is applicable for the analysis of sequence variations of strain collections with more than 10 individual isolates. Pooled sequencing of 36 clinical Pseudomonas aeruginosa isolates revealed that essential and highly expressed proteins evolve at lower rates, wherea

SUBMITTER: Dotsch A 

PROVIDER: S-EPMC2864246 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature