Discovery and comparative profiling of microRNAs in a sweet orange red-flesh mutant and its wild type.

ABSTRACT:

Background

Red-flesh fruit is absent from common sweet orange varieties, but is more preferred by consumers due to its visual attraction and nutritional properties. Our previous researches on a spontaneous red-flesh mutant revealed that the trait is caused by lycopene accumulation and is regulated by both transcriptional and post-transcriptional mechanisms. However, the knowledge on post-transcriptional regulation of lycopene accumulation in fruits is rather limited so far.

Results

We used Illumina sequencing method to identify and quantitatively profile small RNAs on the red-flesh sweet orange mutant and its wild type. We identified 85 known miRNAs belonging to 48 families from sweet orange. Comparative profiling revealed that 51 known miRNAs exhibited significant expressio

SUBMITTER: Xu Q 

PROVIDER: S-EPMC2864249 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature