Basal core promoters control the equilibrium between negative cofactor 2 and preinitiation complexes in human cells.

ABSTRACT:

Background

The general transcription factor TFIIB and its antagonist negative cofactor 2 (NC2) are hallmarks of RNA polymerase II (RNAPII) transcription. Both factors bind TATA box-binding protein (TBP) at promoters in a mutually exclusive manner. Dissociation of NC2 is thought to be followed by TFIIB association and subsequent preinitiation complex formation. TFIIB dissociates upon RNAPII promoter clearance, thereby providing a specific measure for steady-state preinitiation complex levels. As yet, genome-scale promoter mapping of human TFIIB has not been reported. It thus remains elusive how human core promoters contribute to preinitiation complex formation in vivo.

Results

We compare target genes of TFIIB and NC2 in human B cells and analyze associated core promoter archi

SUBMITTER: Albert TK 

PROVIDER: S-EPMC2864573 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature