Dynamic zebrafish interactome reveals transcriptional mechanisms of dioxin toxicity.

ABSTRACT:

Background

In order to generate hypotheses regarding the mechanisms by which 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin) causes toxicity, we analyzed global gene expression changes in developing zebrafish embryos exposed to this potent toxicant in the context of a dynamic gene network. For this purpose, we also computationally inferred a zebrafish (Danio rerio) interactome based on orthologs and interaction data from other eukaryotes.

Methodology/principal findings

Using novel computational tools to analyze this interactome, we distinguished between dioxin-dependent and dioxin-independent interactions between proteins, and tracked the temporal propagation of dioxin-dependent transcriptional changes from a few genes that were altered initially, to large groups of biologicall

SUBMITTER: Alexeyenko A 

PROVIDER: S-EPMC2864754 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature