JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction.

ABSTRACT:

Background and aims

Chronic idiopathic intestinal pseudo-obstruction (CIIP) is characterised by severe impairment of intestinal propulsive motility that mimics bowel obstruction. JC virus (JCV) is a polyomavirus that can infect brain glial cells causing a fatal disease, but may also be found throughout the normal gastrointestinal tract. The hypothesis that JCV infects the myenteric plexuses of patients with CIIP was tested.

Methods

10 patients with CIIP and 61 normal specimens (30 ascending colon and 31 ileum) from patients with uncomplicated colon cancer were studied. DNA was extracted from the myenteric plexuses, and JCV T antigen (TAg) DNA and the viral regulatory region were detected by PCR and sequencing. Immunohistochemistry was performed to detect JCV viral protein ex

SUBMITTER: Selgrad M 

PROVIDER: S-EPMC2865195 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature