Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells.

ABSTRACT: Rapidly emerging techniques of super-resolution single-molecule microscopy of living cells rely on the continued development of genetically encoded photoactivatable fluorescent proteins. On the basis of monomeric TagRFP, we have developed a photoactivatable TagRFP protein that is initially dark but becomes red fluorescent after violet light irradiation. Compared to other monomeric dark-to-red photoactivatable proteins including PAmCherry, PATagRFP has substantially higher molecular brightness, better pH stability, substantially less sensitivity to blue light, and better photostability in both ensemble and single-molecule modes. Spectroscopic analysis suggests that PATagRFP photoactivation is a two-step photochemical process involving sequential one-photon absorbance by two distinct chromop

SUBMITTER: Subach FV 

PROVIDER: S-EPMC2866019 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature