Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumber Holothuria glaberrima.

ABSTRACT: Repair and regeneration are key processes for tissue maintenance, and their disruption may lead to disease states. Little is known about the molecular mechanisms that underline the repair and regeneration of the digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model to dissect and characterize the molecular events during intestinal regeneration. To study the gene expression profile, cDNA libraries were constructed from normal, 3-day, and 7-day regenerating intestines of H. glaberrima. Clones were randomly sequenced and queried against the nonredundant protein database at the National Center for Biotechnology Information. RT-PCR analyses were made of several genes to determine their expression profile during intestinal regeneration. A total of 5,173 sequences

SUBMITTER: Rojas-Cartagena C 

PROVIDER: S-EPMC2866185 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature