Formation of covalently bound protein adducts from the cytotoxicant naphthalene in nasal epithelium: species comparisons.

ABSTRACT:

Background

Naphthalene is a volatile hydrocarbon that causes dose-, species-, and cell type-dependent cytotoxicity after acute exposure and hyperplasia/neoplasia after lifetime exposures in rodents. Toxicity depends on metabolic activation, and reactive metabolite binding correlates with tissue and site susceptibility.

Objectives

We compared proteins adducted in nasal epithelium from rats and rhesus macaques in vitro.

Methods

Adducted proteins recovered from incubations of nasal epithelium and 14C-naphthalene were separated by two-dimensional (2D) gel electrophoresis and imaged to register radioactive proteins. We identified proteins visualized by silver staining on complementary non-radioactive gels by peptide mass mapping.

Results

The levels of reactive metab

SUBMITTER: DeStefano-Shields C 

PROVIDER: S-EPMC2866680 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature