Quantifying fluid shear stress in a rocking culture dish.

ABSTRACT: Fluid shear stress (FSS) is an important stimulus for cell functions. Compared with the well established parallel-plate and cone-and-plate systems, a rocking "see-saw" system offers some advantages such as easy operation, low cost, and high throughput. However, the FSS spatiotemporal pattern in the system has not been quantified. In the present study, we developed a lubrication-based model to analyze the FSS distributions in a rocking rectangular culture dish. We identified an important parameter (the critical flip angle) that dictates the overall FSS behaviors and suggested the right conditions to achieving temporally oscillating and spatially relatively uniform FSS. If the maximal rocking angle is kept smaller than the critical flip angle, which is defined as the angle when the fluid fre

SUBMITTER: Zhou X 

PROVIDER: S-EPMC2866761 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature