Label-free detection of drug-membrane association using ultraviolet-visible sum-frequency generation.

ABSTRACT: Drug-membrane interactions play a crucial role in the pharmacology and activity of drugs. The measurement of drug association to lipid membranes has conventionally been measured by fluorescence and other spectroscopic methods. However, a main disadvantage of fluorescence labeling of drugs is that the introduction of fluorophores may change the molecules physical properties, such as charge, hydrophobic or hydrophilic character, and structure. To circumvent these problems, Ultraviolet-Visible Sum Frequency Generation (UV-Vis SFG) has been developed as an ultrasensitive and label-free technique to detect small-molecule drug association to lipid membranes. Four different classes of drugs, a nonsteroidal anti-inflammatory drug (ibuprofen), antibiotic (azithromycin), antifungal (tolnaftate), and

SUBMITTER: Nguyen TT 

PROVIDER: S-EPMC2867465 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature